Journal: Antioxidants

Article Title: Piperlongumine Induces Cell Cycle Arrest via Reactive Oxygen Species Accumulation and IKKβ Suppression in Human Breast Cancer Cells

doi: 10.3390/antiox8110553

Figure Lengend Snippet: Primers used for real-time PCR.

Article Snippet: An NF-κB inhibitor (Bay 11-7082), and antibodies against cyclin D1, CDK4, CDK6, proliferating cell nuclear antigen (PCNA), NF-κB p65, IκBα, lamin B, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), goat anti-rabbit IgG-HRP, and donkey anti-goat IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques:

Journal: Antioxidants

Article Title: Piperlongumine Induces Cell Cycle Arrest via Reactive Oxygen Species Accumulation and IKKβ Suppression in Human Breast Cancer Cells

doi: 10.3390/antiox8110553

Figure Lengend Snippet: Effects of piperlongumine (PL) on cell cycle-regulatory proteins in MCF-7 cells. The expression of cyclin B1, p-CDK1, CDK1, PCNA, cyclin D1, CDK6, and CDK4 at mRNA ( A ) and protein levels ( B ) was measured using real time PCR and Western blotting, respectively. GAPDH was used as an internal control gene and as a loading control. Data represent the mean ± SEM (n = 3). * Significantly different compared with the control ( p < 0.05).

Article Snippet: An NF-κB inhibitor (Bay 11-7082), and antibodies against cyclin D1, CDK4, CDK6, proliferating cell nuclear antigen (PCNA), NF-κB p65, IκBα, lamin B, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), goat anti-rabbit IgG-HRP, and donkey anti-goat IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Stem Cell Research & Therapy

Article Title: Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation

doi: 10.1186/s13287-019-1139-7

Figure Lengend Snippet: The expression changes of ERK, AKT, p-CREB, c-fos, STAT3, PCNA, Cyclin A2, and Cyclin E after treatment of FGF5. a , b Western blot revealed the expression changes of p-ERK and p-AKT after 100-ng/ml FGF5 treatment. ERK, AKT, and ACTB were used as loading controls. c The expression changes of p-CREB, c-fos, and STAT3 after treatment of FGF5 for 5 and 15 min. d The expression changes of PCNA, Cyclin A2, and Cyclin E after 100 ng/ml FGF5 treatment for 6 and 12 h

Article Snippet: After blocking with 5% milk, membranes were incubated with antibodies against FGF5 (R&D, MAB2371, dilution 1:2000), p-ERK1/2 (CST, 4370, dilution 1:2000), ERK1/2 (CST, 4695, dilution 1:1000), p-AKT (CST, 4060, dilution 1:2000), AKT (CST, 4691, dilution 1:1000), p-CREB (Santa Cruz, sc-81486, dilution 1:1000), c-fos (Santa Cruz, sc-447, dilution 1:600), STAT3 (Santa Cruz, sc-8019, dilution 1:200), PCNA (Santa Cruz, sc-25280, dilution 1:600), Cyclin A2 (Proteintech, 18202-1-AP, dilution 1:2000), Cyclin E1 (Proteintech, 11554-1-AP, dilution 1:2000), or ACTB (beta-actin) (Santa Cruz, sc-47778, dilution 1:500) at 4 °C overnight.

Techniques: Expressing, Western Blot